Nptii Gene Marker Manual

nptii gene marker manual


Besides its use as a selectable marker, the gene is also very attractive for use as a generally applicable indicator gene to examine gene expression and gene regulation (Reiss et al., 1984).

nptii gene marker manual

Use of the tobacco feedback-insensitive anthranilate

Neomycin phosphotransferase gene (nptII) as a selectable marker for Brachypodium BD21 January 2011 Brachypodium distachyon BD21 is an embryogenic diploid accession and …

nptii gene marker manual

8VHU 0DQXDO Software PowerTools for Genetic Analysis

The method is based on a site-specific excision of the nptII marker gene which is mediated by heat-shock inducible expression of the FLP recombinase driven by the soybean GmHsp17.5-E promoter. A monitoring vector containing the nptII and the flp genes in a box flanked by two direct repeated FRT recognition sites was developed.

nptii gene marker manual

Efficient seed-specifically regulated autoexcision of

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Nptii gene marker manual
neo NPTII - Nicotiana tabacum (Common tobacco) - neo
nptii gene marker manual

US5962768A Marker gene - Google Patents

pMR2, which contains the nptII gene without its promoter, was constructed by cloning of the 1.5-kb BglII-BamHI fragment of pKm1 encompassing nptII into the vector pACYC184 (Fig. 1B).

nptii gene marker manual

A Comparison of Two Selectable Marker Gene Systems Used in

antibiotic resistance marker gene, nptII, under the control of a plant promoter. The nptII gene is used for the selection of transformed plants in the laboratory as well as for identification purposes in the field. Apart from its use as a marker in the field, the gene serves no agronomic purpose in the crop. Some concerns are expressed in relation to the use of antibiotic resistance genes in

nptii gene marker manual

Co-transformation using a negative selectable marker gene

The National Research Council of Canada has two granted patents, in the United States and in Europe, directed to dual genetic markers composed of fused genes, which provide a reporter marker gene (glucuronidase (gusA) gene) and an antibiotic resistance gene (nptII gene).

nptii gene marker manual

NPTII Assays for Measuring Gene Expression and Enzyme

DIR 051/2004 - Risk Assessment and Risk Management Plan Executive Summary II kanamycin, neomycin and geneticin and was used as a selectable marker in the laboratory to

nptii gene marker manual

Effect of Spacing and Refuge Crops on Gene Transfer of

marker was the nearest marker to monogerm gene. The distance of between this marker and monogerm locus The distance of between this marker and monogerm locus was estimated to 6.1 rf.

nptii gene marker manual

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For the rescue of nptII genes present in chromosomal plant DNA, the system was adapted to natural transformation, which favours the uptake of linear DNA. When competent Acinetobacter sp. BD413 (formerly A. calcoaceticus) cells containing the mutant nptII gene on a plasmid were transformed with DNA from various transgenic plants carrying nptII as a marker gene (Solanum tuberosum, Nicotiana

nptii gene marker manual

Heat-shock-mediated elimination of the nptII marker gene

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nptii gene marker manual

Evaluation of the uniformity and stability of T-DNA

The results showed that the DT-A gene can be used as a good non-conditional negative selectable marker in both tobacco and rice. Both CaMV 35S promoter- and Ubi1 promoter-driven DT-A genes were effective as non-conditional negative selectable markers in rice.

nptii gene marker manual

A Universal Positive-Negative Selection System for Gene

Among the few available marker genes, the nptII gene encoding the neomycin phosphotransferase II and the uid A (gus) gene, encoding for the β-glucuronidase activity, are commonly used. Control

Nptii gene marker manual - GeneMarker HTS User's Manual SoftGenetics

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